Abstract Kmmig1 as a disrupted mutant of MIG1 encoding a regulator for glucose repression in Kluyveromyces marxianus exhibits a histidine-auxotrophic phenotype.Genome-wide expression analysis revealed that only HIS4 in seven HIS genes for histidine biosynthesis was down-regulated in Kmmig1.Consistently, introduction of HIS4 into Kmmig1 suppressed the requirement Alarms - Alarm Systems of histidine.
Considering the fact that His4 catalyzes four of ten steps Stemless Wine Glass in histidine biosynthesis, K.marxianus has evolved a novel and effective regulation mechanism via Mig1 for the control of histidine biosynthesis.Moreover, RNA-Seq analysis revealed that there were more than 1,000 differentially expressed genes in Kmmig1, suggesting that Mig1 is directly or indirectly involved in the regulation of their expression as a global regulator.